[Frontiers in Bioscience 10, 1693-1706, May 1, 2005] 1693 EVIDENCE FOR SIMULTANEOUS 1Na:1Mg AND PING PONG 2Na:1Mg EXCHANGERS IN RAT THYMOCYTES
نویسندگان
چکیده
1. Abstract 2. Introduction 3. Materials and methods 3.1. Materials and media 3.1.1. Preparation of thymocytes 3.2. Mg loading procedure 3.3. Measurement of intracellular Na and Mg 3.4. Measurement of Nao-induced Mg efflux 3.5. Measurement of Nao-induced Mgit and Nait changes 3.6. Statistical analysis 4. Results 4.1. Nait and Mgit content 4.2. Plasmalemmal Mg leak was not enhanced by A-23187 Mg loading 4.3. Nao-induced Mg efflux in fresh and Mg-loaded thymocytes 4.4. Dependence of Nao-induced Mg efflux on internal Mgit 4.5. Induction of Mgit decrease and Nait increase by Nao in Mg-loaded thymocytes 4.6. Inhibition by amiloride of either Nao-induced Mg efflux and Nao-induced Na influx in Mg-loaded thymocytes 4.7. Dependence of the VMg(Na)max/KNa ratio to the internal Mg content 4.8. Thermodynamics of the Na/Mg exchange 5. Discussion 5.1. Simultaneous versus ping-pong exchange 5.2. Physiological roles of ping pong and simultaneous Na/Mg exchangers coexisting in the same cel 5.3. Appendix: the kinetics of a bisubstrate exchanger 6. Acknowledgments 7. References
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